Medical Subject Headings

Last uploaded: January 16, 2025
Id http://purl.bioontology.org/ontology/MESH/D016133
http://purl.bioontology.org/ontology/MESH/D016133
Preferred Name

Polymerase Chain Reaction

Definitions
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Synonyms
Inverse Polymerase Chain Reaction
PCR, Anchored
Inverse PCR
PCR
Nested PCR
Anchored PCR
PCR, Nested
Nested Polymerase Chain Reaction
Reactions, Polymerase Chain
Polymerase Chain Reactions
Anchored Polymerase Chain Reaction
Reaction, Polymerase Chain
PCR, Inverse
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Type http://www.w3.org/2002/07/owl#Class
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